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Challenge controls are maintained at ≥ 1 x 10 6 colony-forming units (CFU) with a mean particle size (MPS) of 3.0 ± 0.3 µm. The number of bacterial aerosol droplets contacting the filter media is determined by conducting challenge controls without filter media in the test system. The titer of the assay fluid is determined using standard plate count and/or membrane filtration techniques.
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The challenge is delivered for a one-minute interval and sampling through the AGIs is conducted for two minutes to clear the aerosol chamber. The aerosol droplets are collected in all-glass impingers (AGIs) in parallel. aureus is aerosolized and delivered to the filtration media at a constant flow rate of 30 liters per minute (LPM). The test is conducted using Staphylococcus aureus as the challenge organism. The Increased Bacterial Filtration Efficiency test determines the filtration efficiency by comparing the bacterial control counts to test article effluent counts. Increased Challenge Bacterial Filtration Efficiency (BFE) Filtration efficiency measurements can be determined up to >99.9999%. This procedure uses a higher concentration of challenge to be delivered to each test material. Large numbers of material can be evaluated in a relatively short time.īFE testing is commonly performed along with the Differential Pressure (Delta P) test.įor housed filters, we recommend the Increased Challenge method. The BFE procedure is reproducible, and provides a more severe challenge to most filtration devices than would be expected in normal use. The mean particle size can be tightly controlled and is sized using a six-stage viable-particle Andersen sampler, permitting stage-by-stage analysis. It has been used with little or no modification for years and provides a standard reference for comparison of filtration materials. The BFE test offers a number of advantages over other filtration efficiency tests. This allows filtration efficiencies to be reported up to >99.9%. Challenge controls are maintained at 1700 – 3000 colony-forming units (CFU) with a mean particle size (MPS) of 3.0 ± 0.3 µm. The number of bacterial aerosol droplets contacting the filter media is determined by conducting challenge controls without filter medium in the test system. The aerosol droplets are drawn through a six-stage Andersen sampler for collection. aureus is aerosolized and delivered to the filtration media at a constant flow rate of 28.3 liters per minute (LPM) or 1 cubic foot per minute (CFM). After the filtration media is preconditioned, a liquid suspension of S. The Bacterial Filtration Efficiency test determines the filtration efficiency by comparing the bacterial control counts to test article effluent counts. Study Outline Bacterial Filtration Efficiency (BFE)